Anti-Human TNF Recombinant Antibody (TAB-010)

Name: Anti-Human TNF Recombinant Antibody (TAB-010)
SKU: TAB-010
Rating: 5 (1 reviews)

CAT#: TAB-010

Recombinant monoclonal antibody to Human TNF.

Tested Data Gene Expression

Figure 1 Anti-Human TNF Antibody (TAB-010) in WB

Western blot analysis of TAB-010 was performed with TNF-alpha Protein, Human, Recombinant.

TAB-010 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)

ELISA

Figure 2 Anti-Human TNF Antibody (TAB-010) in ELISA

ELISA analysis of TAB-010 was performed by coating with TNF-alpha Protein, Human, Recombinant.

The secondary antibody: HRP-Anti-Human IgG (H+L)

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Normal Tissue Figure 1 Tonsil

Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78469/168112_A_4_8.jpg

RNA Expression Figure 2 RNA cell line category: Group enriched (HDLM-2, NB-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000232810-TNF

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Human

Type
IgG1, κ

Specificity
Tested positive against native human antigen

Species Reactivity
Human

Applications
Suitable for use in ELISA, IP, FC, FuncS, Neut, IF, IHC and most other immunological methods.

Related Disease
Juvenile Idiopathic Arthitis (JIA)

Product Property

Purity
>95% by SDS-PAGE

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha

Gene ID
7124

UniProt ID
P01375

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(Creative Biolabs Cat# TAB-010, RRID: AB_3111739)

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Publications

Peer-reviewed Publications

Our products have been used extensively by scientists all over the world to accelerate their life science research. Here, we present a collection of publications that cite this product.

Nouadje, Georges, and Thierry Ligneel. "Improved immunofixation electrophoresis method with target component on-gel immunodisplacement." U.S. Patent Application No. 16/320,521.

Related Diseases

Asthma

Inflammatory Bowel Diseases

Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-010.pdf

MSDS

MSDS - TAB-010.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
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